5FPL
Crystal structure of human JARID1B in complex with CCT363901
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-22 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 142.910, 142.910, 152.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.170 - 2.350 |
R-factor | 0.192 |
Rwork | 0.191 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a1f |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.170 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.150 | 0.150 |
Number of reflections | 38565 | |
<I/σ(I)> | 14.27 | 14.27 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 20.1 | 19.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.1M HEPES PH 8.0, 0.8M POTASSIUM PHOSPHATE-DIBASIC, 0.8M SODIUM PHOSPHATE MONOBASIC |