5FOG
Crystal structure of hte Cryptosporidium muris cytosolic leucyl-tRNA synthetase editing domain complex with a post-transfer editing analogue of norvaline (Nv2AA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.730, 107.730, 309.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.760 - 2.300 |
| R-factor | 0.20261 |
| Rwork | 0.201 |
| R-free | 0.23607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wfg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.382 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 1.200 |
| Number of reflections | 81817 | |
| <I/σ(I)> | 16 | 1.9 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 8.1 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 0.1 M KNO3 PH 6.9, 22% (W/V) PEG 3350. 20 % ETHYLENE GLYCOL AS CRYOPROTECTANT. |
