5FOB
Crystal Structure of Human Complement C3b in complex with Smallpox Inhibitor of Complement (SPICE)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.891, 83.311, 127.377 |
| Unit cell angles | 75.03, 76.20, 68.50 |
Refinement procedure
| Resolution | 42.356 - 2.600 |
| R-factor | 0.1989 |
| Rwork | 0.198 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2I07 3o8e 2wii |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.390 |
| Number of reflections | 84531 | |
| <I/σ(I)> | 4.3 | 1 |
| Completeness [%] | 97.2 | 96.3 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 75MM AMMONIUM IODIDE, 3.5% PEG 3350 |
