5FKO
TetR(D) E147A mutant in complex with anhydrotetracycline and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.467, 68.467, 178.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.020 - 1.850 |
| R-factor | 0.21293 |
| Rwork | 0.211 |
| R-free | 0.25993 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d7m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.183 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.600 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.670 |
| Number of reflections | 18638 | |
| <I/σ(I)> | 29.6 | 2.1 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 8.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 295 | 15% PEG 4000, 100 MM MES PH 6.5, 600 MM NACL, 1 MM 5A,6-ANHYDROTETRACYCLINE, 7.5 MM MGCL2 AT 295 K |
