5FIR

Crystal structure of C. elegans XRN2 in complex with the XRN2-binding domain of PAXT-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	170.210, 200.770, 202.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.393 - 2.836
R-factor	0.1867
Rwork	0.185
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HOMOLOGY MODEL
RMSD bond length	0.011
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.910
High resolution limit [Å]	2.840	2.840
Rmerge	0.130	1.260
Number of reflections	155519
<I/σ(I)>	7.1	0.75
Completeness [%]	94.6	84.5
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				2M AMMONIUM SULFATE, 0.1 M BIS-TRIS PH 6.5