5FIC
Open form of murine Acid Sphingomyelinase in presence of lipid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-11 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97959 |
Spacegroup name | P 41 |
Unit cell lengths | 101.750, 101.750, 401.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.318 - 2.800 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.23560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hnq |
RMSD bond length | 0.005 |
RMSD bond angle | 0.640 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.318 |
High resolution limit [Å] | 2.800 |
Number of reflections | 99971 |
<I/σ(I)> | 9.92 |
Completeness [%] | 100.0 |
Redundancy | 6.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium/potassium phosphate, MES, sodium chloride |