5FFY
Crystal structure of the bromodomain of human BRPF1 in complex with a benzimidazole ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 48.545, 59.869, 51.236 |
| Unit cell angles | 90.00, 100.43, 90.00 |
Refinement procedure
| Resolution | 23.880 - 1.550 |
| R-factor | 0.18475 |
| Rwork | 0.183 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.633 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.190 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.037 | 0.402 |
| Number of reflections | 20656 | |
| <I/σ(I)> | 19.1 | 3.1 |
| Completeness [%] | 98.5 | 97.7 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277.15 | 0.1 M bis tris propane pH 9, 10% ethylene glycol, 0.15 M sodium nitrate |
