5FCW
HDAC8 Complexed with a Hydroxamic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.443, 84.548, 94.307 |
| Unit cell angles | 90.00, 100.08, 90.00 |
Refinement procedure
| Resolution | 49.659 - 1.979 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.670 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.061 |
| Number of reflections | 107583 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 97.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | 4% PEG 3350, 50 mM buffer (MES, pH 5.3) |
