5FB0

Crystal Structure of a PHD finger bound to histone H3 T3ph peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	83.830, 99.160, 53.716
Unit cell angles	90.00, 93.17, 90.00

Refinement procedure

Resolution	32.143 - 2.702
R-factor	0.2289
Rwork	0.224
R-free	0.27610
RMSD bond length	0.003
RMSD bond angle	0.635
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10_2155: ???)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.136	0.056	0.725
Rmeas	0.151	0.062	0.800
Rpim	0.064	0.026	0.336
Total number of observations	67541
Number of reflections	12044
<I/σ(I)>	7.5
Completeness [%]	99.9	98.7	100
Redundancy	5.6	5.4	5.7
CC(1/2)		0.996	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG 3350, 0.2 M sodium citrate