5FAE
N184K pathological variant of gelsolin domain 2 (trigonal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.919, 46.919, 84.623 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.630 - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.182 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kcq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.538 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.630 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.104 | |
| Number of reflections | 12405 | |
| <I/σ(I)> | 7.5 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M lithium sulfate 0.1 M tris 40% PEG 400 |
