5F9H
Crystal structure of RIG-I helicase-RD in complex with 24-mer 5' triphosphate hairpin RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.180, 174.810, 309.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.442 - 3.100 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.26560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e3h |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.737 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.664 | 3.400 |
| High resolution limit [Å] | 3.100 | 3.200 |
| Rmerge | 0.280 | |
| Number of reflections | 109315 | |
| <I/σ(I)> | 9.6 | 2.4 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 11.1 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23% (w/v) PEG 3350, 0.25 M KSCN, 100 mM MOPS (pH 7.8) |
