5F6B
Crystal structure of the Q108K:K40L:T51V:R58Y:Y19W mutant of human Cellular Retinol Binding Protein II in complex with All-trans-Retinal at 1.3 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 |
Unit cell lengths | 29.953, 35.721, 64.937 |
Unit cell angles | 90.85, 88.45, 65.68 |
Refinement procedure
Resolution | 28.760 - 1.310 |
R-factor | 0.2031 |
Rwork | 0.201 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4exz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.146 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.330 |
High resolution limit [Å] | 1.310 | 1.310 |
Rmerge | 0.036 | 0.410 |
Number of reflections | 59171 | |
<I/σ(I)> | 38.98 | 1.9 |
Completeness [%] | 94.6 | 89.2 |
Redundancy | 3.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 25 %PEG 4000, 0.1 M Ammonium Acetate, 0.1 M Sodium Acetate pH = 4.5. |