5F5P
Molecular Basis for Shroom2 Recognition by Rock1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.587, 133.795, 135.871 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 3.568 |
R-factor | 0.2732 |
Rwork | 0.271 |
R-free | 0.28690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3thf |
RMSD bond length | 0.002 |
RMSD bond angle | 0.538 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2219)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.570 | 3.570 |
Rmerge | 0.080 | |
Number of reflections | 21121 | |
<I/σ(I)> | 24.5 | 1.72 |
Completeness [%] | 97.8 | 97.9 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 3350, 200 mM Magnesium Chloride, 100 mM Tris pH 8.5 |