5F2U
Structure of Fully modified farnesylated INPP5E Peptide in complex with PDE6D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.007 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.470, 81.200, 117.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.530 - 1.850 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.20750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t5g |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.011 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (5.7.0032) |
| Phasing software | MOLREP (3.15) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.530 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.097 | 0.672 |
| Number of reflections | 30251 | |
| <I/σ(I)> | 10.42 | 3.09 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.39 | 6.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.4 M sodium malonate |
