5F1G

Crystal structure of AmpC BER adenylylated in the cytoplasm

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	60.478, 65.414, 106.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.707 - 1.760
R-factor	0.1726
Rwork	0.172
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3b
RMSD bond length	0.007
RMSD bond angle	1.142
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.760
Number of reflections	42395
<I/σ(I)>	79.18
Completeness [%]	99.6
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	3.2	295	LiSO4, Citric Acid, PEG 3350, MgCl2