5EWM

CRYSTAL STRUCTURE OF AMINO TERMINAL DOMAINS OF THE NMDA RECEPTOR SUBUNIT GLUN1 AND GLUN2B IN COMPLEX WITH EVT-101

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	268.400, 60.610, 144.500
Unit cell angles	90.00, 116.27, 90.00

Refinement procedure

Resolution	40.410 - 2.760
R-factor	0.1728
Rwork	0.171
R-free	0.21090
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5ewj
RMSD bond length	0.010
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	Aimless (0.3.5)
Phasing software	BUSTER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	129.570	129.570	3.900
High resolution limit [Å]	2.760	3.900	2.760
Rmerge	0.055	0.033	0.140
Rpim	0.036	0.021	0.089
Total number of observations	177662	59953	117709
Number of reflections	53496
<I/σ(I)>	14.1	25.9	7.6
Completeness [%]	98.3	96.4	99.2
Redundancy	3.3	3.2	3.4
CC(1/2)	0.999	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	3.6M Na Formate, 0.1 M Hepes pH 7.0