5EV1

Structure I of Intact U2AF65 Recognizing a 3' Splice Site Signal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	C 2 2 21
Unit cell lengths	62.052, 114.241, 59.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.456 - 2.037
R-factor	0.1778
Rwork	0.173
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g4b
RMSD bond length	0.013
RMSD bond angle	1.320
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.456	32.456	2.150
High resolution limit [Å]	2.037	6.440	2.037
Rmerge	0.039	0.022	0.321
Rmeas	0.044
Rpim	0.019	0.011	0.171
Total number of observations	61150	2093	6269
Number of reflections	13202
<I/σ(I)>	21.2	50.9	4.2
Completeness [%]	95.5	95.7	78.3
Redundancy	4.6	4.4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM phosphate-citrate buffer, 40% Peg400, 10% dioxane