5EU7
Crystal structure of HIV-1 integrase catalytic core in complex with Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2009-11-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.489, 87.650, 96.339 |
Unit cell angles | 90.00, 95.83, 90.00 |
Refinement procedure
Resolution | 95.840 - 2.640 |
R-factor | 0.18401 |
Rwork | 0.181 |
R-free | 0.23317 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1EX4 and 1FVD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.624 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.840 | 2.729 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmerge | 0.064 | 0.156 |
Number of reflections | 42891 | |
<I/σ(I)> | 11.54 | 5.92 |
Completeness [%] | 98.7 | 92.99 |
Redundancy | 7.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 100mM BisTrisPropane pH 7.0 |