5EU6
HLA Class I antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.520, 54.410, 112.120 |
| Unit cell angles | 85.01, 81.64, 72.63 |
Refinement procedure
| Resolution | 51.870 - 2.020 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i4w |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.964 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.873 | 51.873 | 2.070 |
| High resolution limit [Å] | 2.020 | 9.030 | 2.020 |
| Rmerge | 0.052 | 0.396 | |
| Rmeas | 0.087 | ||
| Rpim | 0.057 | 0.032 | 0.398 |
| Total number of observations | 128191 | 1491 | 8955 |
| Number of reflections | 64983 | ||
| <I/σ(I)> | 5.5 | 9.9 | 1.9 |
| Completeness [%] | 97.7 | 97.3 | 96.7 |
| Redundancy | 2 | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.2 M sodium sulphate, 0.1 M Bis Tris propane pH 6.5, 20% w/v PEG 3350. |
