5EU4
HLA Class I antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 56.080, 57.630, 79.930 |
Unit cell angles | 90.00, 89.81, 63.84 |
Refinement procedure
Resolution | 43.390 - 2.120 |
R-factor | 0.1736 |
Rwork | 0.172 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i4w |
RMSD bond length | 0.021 |
RMSD bond angle | 2.067 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.12) |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.390 | 43.390 | 2.180 |
High resolution limit [Å] | 2.120 | 9.480 | 2.120 |
Rmerge | 0.092 | 0.050 | 0.427 |
Rpim | 0.084 | 0.045 | 0.392 |
Total number of observations | 99386 | 1155 | 7463 |
Number of reflections | 49667 | ||
<I/σ(I)> | 6.5 | 14.4 | 2.3 |
Completeness [%] | 97.4 | 93.3 | 96.7 |
Redundancy | 2 | 2.2 | 2.1 |
CC(1/2) | 0.985 | 0.984 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Tris pH 8.0, 15 % Glycerol, 20 % PEG 4000 |