5EMJ
Crystal structure of PRMT5:MEP50 with Compound 8 and sinefungin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-07-19 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97856 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 104.240, 138.830, 179.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.050 - 2.273 |
R-factor | 0.2115 |
Rwork | 0.209 |
R-free | 0.26590 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.442 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.20) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 69.415 | 69.415 | 2.330 |
High resolution limit [Å] | 2.273 | 10.170 | 2.270 |
Rmerge | 0.017 | 0.781 | |
Rmeas | 0.059 | ||
Rpim | 0.026 | 0.009 | 0.387 |
Total number of observations | 299240 | 2991 | 21908 |
Number of reflections | 59262 | ||
<I/σ(I)> | 19.9 | 68.4 | 2.1 |
Completeness [%] | 99.0 | 93.2 | 99.6 |
Redundancy | 5 | 4.3 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium acetate, 0.1 M Sodium citrate pH 6.1, 10% w/v PEG 4000 |