5EKD
Human mitochondrial tryptophanyl-tRNA synthetase bound by indolmycin and Mn*ATP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.289, 78.087, 153.996 |
| Unit cell angles | 90.00, 96.55, 90.00 |
Refinement procedure
| Resolution | 29.060 - 1.820 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dk4 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.912 |
| Data scaling software | SCALA |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.060 | 1.885 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.077 | 0.594 |
| Number of reflections | 60563 | |
| <I/σ(I)> | 8.1 | 1.5 |
| Completeness [%] | 98.5 | 94.08 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | sodium acetate, glycerol, peg 5000 |
