5EJK

Crystal structure of the Rous sarcoma virus intasome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	124.936, 157.854, 126.583
Unit cell angles	90.00, 110.94, 90.00

Refinement procedure

Resolution	49.215 - 3.800
R-factor	0.2557
Rwork	0.254
R-free	0.29450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fw1 1k6y
RMSD bond length	0.008
RMSD bond angle	0.715
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2210: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.248
High resolution limit [Å]	3.610
Rmerge		0.147
Number of reflections	52483
<I/σ(I)>	9.6
Completeness [%]	99.0
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	Sodium formate