5EI3

Co-crystal structure of eIF4E with nucleotide mimetic inhibitor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.85
Spacegroup name	P 21 2 21
Unit cell lengths	38.590, 52.080, 125.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.035 - 1.710
R-factor	0.1667
Rwork	0.166
R-free	0.18730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v8w
RMSD bond length	0.007
RMSD bond angle	0.946
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.595	40.035	1.800
High resolution limit [Å]	1.710	5.410	1.710
Rmerge		0.039	0.342
Rmeas	0.102
Rpim	0.039	0.017	0.138
Total number of observations	195799	6387	28759
Number of reflections	28165
<I/σ(I)>	15.7	36.2	5.3
Completeness [%]	100.0	99.5	100
Redundancy	7	6.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	21-31% PEG 8000, 1???3% (NH4)2SO4, 100 mM HEPES, pH 7.5, 1 round of seeding