5EH2
Human PRDM9 allele-A ZnF Domain with Associated Recombination Hotspot DNA Sequence III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-10 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.208, 57.756, 76.206 |
| Unit cell angles | 90.15, 75.30, 83.30 |
Refinement procedure
| Resolution | 32.747 - 2.050 |
| R-factor | 0.2022 |
| Rwork | 0.201 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Zn SAD determined structure |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.750 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.042 | 0.456 |
| Number of reflections | 41966 | |
| <I/σ(I)> | 13.63 | 2.2 |
| Completeness [%] | 90.4 | 76.8 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.058 M Bistris Propane, 0.042 M Citric acid, 20% PEG3350 |
