5EGS
Human PRMT6 with bound fragment-type inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.157, 135.616, 83.086 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 41.260 - 2.150 |
| R-factor | 0.20174 |
| Rwork | 0.201 |
| R-free | 0.24648 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hc4 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.134 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.145 | 0.775 |
| Number of reflections | 90370 | |
| <I/σ(I)> | 8.4 | 1.7 |
| Completeness [%] | 99.2 | 96.8 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25 % PEG 1500; 0.1 M MMT pH 8.0 |
