5EGO
HOXB13-MEIS1 heterodimer bound to methylated DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.984000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.976, 51.480, 113.575 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.544 - 2.540 |
R-factor | 0.2296 |
Rwork | 0.228 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xrm |
RMSD bond length | 0.006 |
RMSD bond angle | 1.065 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.630 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.048 | 0.761 |
Number of reflections | 8290 | |
<I/σ(I)> | 15.5 | 1.6 |
Completeness [%] | 98.7 | 98.8 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 5000, potassium chloride, magnesium chloride, Tris, PEG 400 |