5EFQ
Crystal structure of human Cdk13/Cyclin K in complex with ADP-aluminum fluoride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.780, 81.820, 93.140 |
| Unit cell angles | 74.08, 84.26, 76.89 |
Refinement procedure
| Resolution | 46.186 - 2.000 |
| R-factor | 0.1966 |
| Rwork | 0.194 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nst |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.121 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | |
| High resolution limit [Å] | 2.000 | |
| Number of reflections | 90134 | |
| <I/σ(I)> | 13 | 1.7 |
| Completeness [%] | 96.0 | |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris, pH 6.5, 25.5% PEG 3350, 0.35 M MgCl2 |
