5EDW

Ternary structure of Dpo4 bound to G in the template base paired with incoming dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 21 21 2
Unit cell lengths	98.516, 101.994, 52.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.975 - 2.620
R-factor	0.2228
Rwork	0.220
R-free	0.28420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s0n
RMSD bond length	0.005
RMSD bond angle	0.755
Data reduction software	DENZO
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.670
High resolution limit [Å]	2.620	2.620
Rmerge	0.097
Number of reflections	16600
<I/σ(I)>	23.25	1.82
Completeness [%]	99.9
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	295	12 to 18% PEG 3350, 100 mM HEPES, 100 mM calcicum acetate, and 2.5% glycerol