5EDM
Crystal structure of prothrombin deletion mutant residues 154-167 ( Form I )
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-08-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.884, 168.690, 144.315 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.070 - 2.200 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 4O03 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.681 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.070 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.087 | 0.487 |
| Number of reflections | 67055 | |
| <I/σ(I)> | 17 | 3 |
| Completeness [%] | 98.0 | 97.8 |
| Redundancy | 7.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES, pH 6.5 and 1.6 M MgSO4 |
