5ED2

Human Adenosine Deaminase Acting on dsRNA (ADAR2) mutant E488Q bound to dsRNA sequence derived from human GLI1 gene

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	79.130, 81.614, 256.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.565 - 2.950
R-factor	0.1889
Rwork	0.188
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zy7
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.030
High resolution limit [Å]	2.950	2.950
Rmerge	0.096	1.351
Number of reflections	35355
<I/σ(I)>	12.27	1.16
Completeness [%]	98.1	98.9
Redundancy	5.19	5.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M MES/NaOH, pH 6.5, 12% PEG20000