5ED1

Human Adenosine Deaminase Acting on dsRNA (ADAR2) mutant E488Q bound to dsRNA sequence derived from S. cerevisiae BDF2 gene

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	82.362, 107.498, 121.097
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.392 - 2.770
R-factor	0.1659
Rwork	0.163
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zy7
RMSD bond length	0.010
RMSD bond angle	1.342
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.820
High resolution limit [Å]	2.750	2.750
Rmerge	0.070	0.685
Number of reflections	27727
<I/σ(I)>	11.57	1.52
Completeness [%]	96.5	98.8
Redundancy	2.93	3.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M MES/NaOH, pH 6.5, 9% w/v PEG3350, 13% glycerol, 0.015 M NAD