5ECT
Mycobacterium tuberculosis dUTPase G143STOP mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8729 |
| Spacegroup name | P 63 |
| Unit cell lengths | 54.780, 54.780, 84.079 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.040 - 1.300 |
| R-factor | 0.12441 |
| Rwork | 0.123 |
| R-free | 0.15679 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3hza |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.899 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.050 | 1.344 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.028 | |
| Number of reflections | 34842 | |
| <I/σ(I)> | 21.23 | 1.95 |
| Completeness [%] | 99.1 | 99.27 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5 M ammonium sulfate, 0.1M Tris/HCl, 12% glycerol |
