5EC7
Crystal structure of a chimeric c-Src-SH3 domain with the sequence of the RT-loop from the Abl-SH3 domain at pH 5.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.472, 42.348, 52.579 |
Unit cell angles | 90.00, 90.47, 90.00 |
Refinement procedure
Resolution | 19.640 - 1.650 |
R-factor | 0.1933 |
Rwork | 0.191 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jz4 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.932 |
Data reduction software | autoPROC |
Data scaling software | Aimless (0.5.12) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.640 | 19.640 | 1.680 |
High resolution limit [Å] | 1.650 | 8.890 | 1.650 |
Rmerge | 0.054 | 0.027 | 0.439 |
Rpim | 0.039 | 0.018 | 0.310 |
Total number of observations | 59267 | 367 | 3116 |
Number of reflections | 21249 | ||
<I/σ(I)> | 11.7 | 27.4 | 2.3 |
Completeness [%] | 95.7 | 87.4 | 96.2 |
Redundancy | 2.8 | 2.6 | 2.9 |
CC(1/2) | 0.998 | 0.998 | 0.746 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 2.5 M ammonium sulphate, 5% PEG 300, 2 % Glycerol, 0.1 M sodium acetate |