5E72

Crystal structure of the archaeal tRNA m2G/m22G10 methyltransferase (aTrm11) in complex with S-adenosyl-L-methionine (SAM) from Thermococcus kodakarensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	47.602, 53.688, 134.787
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.674 - 1.739
R-factor	0.1887
Rwork	0.187
R-free	0.22310
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.101
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1682)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.800
High resolution limit [Å]	1.739	3.750	1.740
Rmerge	0.043	0.023	0.217
Total number of observations	185983
Number of reflections	36188
<I/σ(I)>	16.9
Completeness [%]	99.7	98.7	99.3
Redundancy	5.1	4.8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	4.5M NaCl, 0.1M Tris-HCl