5E5A
Crystal structure of the chromatin-tethering domain of Human cytomegalovirus IE1 protein bound to the nucleosome core particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0308 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.700, 109.474, 181.976 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.809 |
R-factor | 0.1978 |
Rwork | 0.196 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 1AOI |
RMSD bond length | 0.008 |
RMSD bond angle | 0.947 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.020 | 2.800 |
Rmerge | 0.133 | 0.076 | 0.611 |
Rmeas | 0.144 | 0.083 | 0.664 |
Rpim | 0.056 | 0.034 | 0.256 |
Total number of observations | 346849 | ||
Number of reflections | 52496 | ||
<I/σ(I)> | 5 | ||
Completeness [%] | 99.9 | 99.7 | 100 |
Redundancy | 6.6 | 6.2 | 6.6 |
CC(1/2) | 0.984 | 0.856 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 289 | sodium cacodylate, magnesium acetate, 2-methyl-2,4-pentanediol |