5E24
Structure of the Su(H)-Hairless-DNA Repressor Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2012-02-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.710, 93.890, 154.390 |
| Unit cell angles | 90.00, 109.83, 90.00 |
Refinement procedure
| Resolution | 31.300 - 2.140 |
| R-factor | 0.1738 |
| Rwork | 0.173 |
| R-free | 0.19260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3brg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.300 | 2.200 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.650 | |
| Number of reflections | 131128 | |
| <I/σ(I)> | 12.5 | 3.1 |
| Completeness [%] | 99.6 | 94.82 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 277 | Tris, PEG3350, (NH4)2SO4 |
