5E24

Structure of the Su(H)-Hairless-DNA Repressor Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	200
Detector technology	CCD
Collection date	2012-02-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	177.710, 93.890, 154.390
Unit cell angles	90.00, 109.83, 90.00

Refinement procedure

Resolution	31.300 - 2.140
R-factor	0.1738
Rwork	0.173
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3brg
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.300	2.200
High resolution limit [Å]	2.140	2.140
Rmerge		0.650
Number of reflections	131128
<I/σ(I)>	12.5	3.1
Completeness [%]	99.6	94.82
Redundancy	7.6	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8	277	Tris, PEG3350, (NH4)2SO4