5E24

Structure of the Su(H)-Hairless-DNA Repressor Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Maltose-binding periplasmic protein	polymer	370	40448.8	2	UniProt (P0AEY0) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli O157:H7	MBP,MMBP,Maltodextrin-binding protein
2	B, D	Protein hairless	polymer	38	4335.0	2	UniProt (Q02308) In PDB	Drosophila melanogaster (Fruit fly)
3	E, F	Suppressor of hairless protein	polymer	424	48040.8	2	UniProt (P28159) Pfam (PF09271) Pfam (PF09270) Pfam (PF20144) In PDB	Drosophila melanogaster (Fruit fly)	J kappa-recombination signal-binding protein,RBP-J kappa
4	G	DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3')	polymer	15	4503.9	1		Mus musculus
5	H	DNA (5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3')	polymer	15	4673.1	1		Mus musculus
6	I, J	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		666.6	2	In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ)		alpha-maltotetraose
7	A, E, F, C, D	1,2-ETHANEDIOL	non-polymer		62.1	113	Chemie (EDO)
8		water	water		18.0	669	Chemie (HOH)

Sequence modifications

A, C: 1 - 370 (UniProt: P0AEY0)

PDB	External Database	Details
Ala 82	Asp 108	engineered mutation
Ala 83	Lys 109	engineered mutation
Ala 172	Glu 198	engineered mutation
Ala 173	Asn 199	engineered mutation
Ala 239	Lys 265	engineered mutation
Asn 367	Arg 393	engineered mutation

E, F: 99 - 522 (UniProt: P28159)

PDB	External Database	Details
Thr 155	Arg 155	engineered mutation
Gly 281	Asn 281	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	194826.2
Branched	Number of molecules	2
	Total formula weight	1333.2
Non-Polymers*	Number of molecules	113
	Total formula weight	7013.7
All*	Total formula weight	203173.1

*Water molecules are not included.