5E0U
Human PCNA variant (S228I) complexed with p21 at 1.9 Angstroms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 3 |
Unit cell lengths | 143.295, 143.295, 41.402 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.439 - 1.930 |
R-factor | 0.1699 |
Rwork | 0.162 |
R-free | 0.22110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1axc |
RMSD bond length | 0.016 |
RMSD bond angle | 1.592 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.440 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.090 | 0.568 |
Number of reflections | 71746 | |
<I/σ(I)> | 20.7 | 2.96 |
Completeness [%] | 99.8 | 98.65 |
Redundancy | 7.7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM HEPES pH 7.5, 25% PEG 3350 |