5E0K
X-ray crystal structure of tryptophan synthase complex from Pyrococcus furiosus at 2.76 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.862, 225.014, 296.241 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.910 - 2.760 |
R-factor | 0.20594 |
Rwork | 0.204 |
R-free | 0.23774 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.172 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.810 |
High resolution limit [Å] | 2.760 | 2.760 |
Number of reflections | 150514 | |
<I/σ(I)> | 8 | 0.6 |
Completeness [%] | 99.3 | 93.7 |
Redundancy | 6.7 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | 16-20 % PEG3350 and 0.15-0.2 M Na citrate |