5DXA
Crystal structure of CARM1, sinefungin, and methylated PABP1 peptide (R460)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 75.179, 98.693, 207.483 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.560 - 2.070 |
R-factor | 0.2005 |
Rwork | 0.198 |
R-free | 0.24400 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.381 |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 2.190 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.119 | 0.709 |
Number of reflections | 94364 | |
<I/σ(I)> | 10.7 | 2.2 |
Completeness [%] | 98.9 | 98.9 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350 |