5DUO
Crystal structure of native translocator protein 18kDa (TSPO) from Rhodobacter sphaeroides (A139T Mutant) in C2 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.003 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 58.388, 99.191, 95.326 |
Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
Resolution | 46.984 - 2.400 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.24430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.151 |
Data reduction software | XDS |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.433 |
High resolution limit [Å] | 2.400 | 2.349 |
Rmerge | 0.140 | 1.127 |
Number of reflections | 22331 | |
<I/σ(I)> | 8.68 | 1.15 |
Completeness [%] | 100.0 | 90 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | 31% v/v pentaerythritol ethoxylate 15/04, 100 mM Tris, pH 7.0, 200 mM ammonium acetate |