5DQ8
Crystal structure of human transcription factor TEAD2 in complex with flufenamic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.819, 61.446, 80.416 |
| Unit cell angles | 90.00, 117.55, 90.00 |
Refinement procedure
| Resolution | 33.661 - 2.305 |
| R-factor | 0.1735 |
| Rwork | 0.171 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l15 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.553 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1951)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 20054 | |
| <I/σ(I)> | 17.2 | 3.39 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1 M HEPES pH 7.2, 2.4 M sodium formate |
