5DNN
Nucleosome core particle containing adducts of gold(I)-triethylphosphane and ruthenium(II)-toluene PTA complexes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2013-09-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.5 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.726, 109.948, 182.041 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.870 - 2.800 |
R-factor | 0.21574 |
Rwork | 0.215 |
R-free | 0.23648 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dnm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.459 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.870 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.037 | 0.433 |
Number of reflections | 51082 | |
<I/σ(I)> | 23.2 | 1.6 |
Completeness [%] | 95.8 | 76.6 |
Redundancy | 5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 40 mM MnCl2, 30 mM KCl, 20 mM K-Cacodylate |