5DM7
Crystal structure of the 50S ribosomal subunit from Deinococcus radiodurans in complex with hygromycin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2012-09-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 169.820, 411.540, 695.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.020 - 3.000 |
| R-factor | 0.2859 |
| Rwork | 0.284 |
| R-free | 0.32550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.030 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.215 | 5.623 |
| Number of reflections | 479454 | |
| <I/σ(I)> | 6.28 | 0.33 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | HEPES, sodium hydroxide, magnesium chloride, ammonium chloride, 2-mercaptoethanol, spermidine, 2-ethyl-1,3-hexanediol, ethanol |
