5DM7
Crystal structure of the 50S ribosomal subunit from Deinococcus radiodurans in complex with hygromycin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2012-09-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00001 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.820, 411.540, 695.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.020 - 3.000 |
R-factor | 0.2859 |
Rwork | 0.284 |
R-free | 0.32550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.215 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.030 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.215 | 5.623 |
Number of reflections | 479454 | |
<I/σ(I)> | 6.28 | 0.33 |
Completeness [%] | 99.5 | 99.1 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | HEPES, sodium hydroxide, magnesium chloride, ammonium chloride, 2-mercaptoethanol, spermidine, 2-ethyl-1,3-hexanediol, ethanol |