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5DM7

Crystal structure of the 50S ribosomal subunit from Deinococcus radiodurans in complex with hygromycin A

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]90
Detector technologyPIXEL
Collection date2012-09-10
DetectorPSI PILATUS 6M
Wavelength(s)1.00001
Spacegroup nameI 2 2 2
Unit cell lengths169.820, 411.540, 695.650
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution57.020 - 3.000
R-factor0.2859
Rwork0.284
R-free0.32550
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.215
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHENIX
Refinement softwarePHENIX
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]59.0303.160
High resolution limit [Å]3.0003.000
Rmerge0.2155.623
Number of reflections479454
<I/σ(I)>6.280.33
Completeness [%]99.599.1
Redundancy4.64.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP292HEPES, sodium hydroxide, magnesium chloride, ammonium chloride, 2-mercaptoethanol, spermidine, 2-ethyl-1,3-hexanediol, ethanol

217705

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