5DKW
Ternary crystal structure of polymerase lambda with a GA mispair at the primer terminus with Ca2+ in the active
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-04-16 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.466, 151.310, 83.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.472 - 2.689 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xsl |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.853 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.790 |
| High resolution limit [Å] | 2.689 | 5.780 | 2.690 |
| Rmerge | 0.143 | 0.049 | 0.818 |
| Rmeas | 0.155 | 0.055 | 0.885 |
| Rpim | 0.058 | 0.023 | 0.335 |
| Total number of observations | 250577 | ||
| Number of reflections | 35654 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 99.6 | 99.4 | 96.6 |
| Redundancy | 7 | 6 | 6.9 |
| CC(1/2) | 0.998 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M HEPES, 30%(v/v) Jeffamine ED-2100 Reagent |
