5DKW

Ternary crystal structure of polymerase lambda with a GA mispair at the primer terminus with Ca2+ in the active

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	95
Detector technology	CCD
Collection date	2014-04-16
Detector	RAYONIX MX300HE
Wavelength(s)	0.90000
Spacegroup name	P 21 21 2
Unit cell lengths	99.466, 151.310, 83.606
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.472 - 2.689
R-factor	0.1865
Rwork	0.184
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsl
RMSD bond length	0.004
RMSD bond angle	0.853
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.790
High resolution limit [Å]	2.689	5.780	2.690
Rmerge	0.143	0.049	0.818
Rmeas	0.155	0.055	0.885
Rpim	0.058	0.023	0.335
Total number of observations	250577
Number of reflections	35654
<I/σ(I)>	8.4
Completeness [%]	99.6	99.4	96.6
Redundancy	7	6	6.9
CC(1/2)		0.998	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1 M HEPES, 30%(v/v) Jeffamine ED-2100 Reagent