5DKP
Crystal Structure of N. meningitidis ClpP in complex with agonist ADEP A54556.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2013-08-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.640, 198.850, 144.040 |
| Unit cell angles | 90.00, 97.81, 90.00 |
Refinement procedure
| Resolution | 42.400 - 2.381 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u0g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.289 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.700 | 2.590 |
| High resolution limit [Å] | 2.380 | 2.390 |
| Rmerge | 0.173 | |
| Number of reflections | 259622 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 50 mM TRIS/HCl, pH 7.5, 200 mM KCl, 1 mM DTT, 10% glycerol |
