5DD0

Crystal structures in an anti-HIV antibody lineage from immunization of Rhesus macaques

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2014-04-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	147.332, 80.406, 37.861
Unit cell angles	90.00, 102.72, 90.00

Refinement procedure

Resolution	24.373 - 2.488
R-factor	0.2322
Rwork	0.227
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q6g 2qqn
RMSD bond length	0.003
RMSD bond angle	0.718
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	15105
<I/σ(I)>	6.9
Completeness [%]	100.0
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24% PEG 1500, 20% glycerol, with seeding