5DBX
Crystal structure of murine SPAK(T243D) in complex with AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-31 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.442, 101.736, 104.077 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.329 - 2.500 |
R-factor | 0.2083 |
Rwork | 0.206 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c30 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.027 |
Data reduction software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.043 | 0.410 |
Number of reflections | 27322 | |
<I/σ(I)> | 31.4 | |
Completeness [%] | 93.0 | |
Redundancy | 11.28 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Mg(OAc)2, 0.1 M Tris (pH 8.5), 16% PEG3350 (Hampton), 0.01 M Na- (CH3)2AsO2, and 0.01 M CaCl2 |