5D7O
Crystal structure of Sirt2-ADPR at an improved resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.000, 78.170, 114.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.710 - 1.630 |
| R-factor | 0.17192 |
| Rwork | 0.171 |
| R-free | 0.19070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zgv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.149 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.710 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.114 | 0.983 |
| Number of reflections | 87718 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 277 | 0.2 M sodium chloride, 30 % (w/v) PEG 3350, 0.1 M Bis-Tris |
